Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil

Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil

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Ent-kaurene oxidase (EKO) catalyze three sequential oxidations in the early steps of gibberellin biosynthesis pathway.In this research, a cDNA sequence of InEKO1 gene in the model short-day plant Ipomoea nil was identified.Our Cleaner studies revealed that inductive conditions for flowering caused an increase in the transcriptional activity of the examined gene in the cotyledons-the main organs for the perception of the photoperiodic stimulus.

In contrast, in the second half of the 16 h long inductive night and after that, a decreased amount of InEKO1 mRNA in the apexes was detected.What is more, ethylene, the key inhibitor of flower induction in I.nil, elevated the InEKO1 expression exclusively in the Floor - Nets cotyledons between 10 and 14 h of the inductive night.